Azure Virtual Machine for Metabarcoding

To assist Cefas staff in conducting metabarcoding studies we have developed a Virtual Machine hosted on Azure. This is equipped with all the software we think you will need to run your analyses without you needing to download software yourself. It includes an installation of Galaxy which hosts many useful programs and allows you to use a graphical interface rather than the command line.

GUI - graphical user interface

If you are planning to use the Graphical user interface then we recommend the use of Frogs. This is hosted within Galaxy. Frogs combines multiple metabarcoding tools into one, and is designed to be user friendly.

Command-line

If you would like to stick to the command line, we would recommend using either Dada2 or Dadaist2, depending on how comfortable you are with each program.

— Author: Nicola Coyle, David Ryder 25/01/2022